human fus Search Results


human fus  (Sino Biological)
90
Sino Biological human fus
Arg and poly-arg additives worked similarly for regulating <t>FUS</t> cluster formation in cultured dopaminergic neurons. ( A ) Effect of Arg and poly-Arg additives to FUS cluster formation in the cells detected by a confocal laser scanning microscopy. Cells were transfected <t>with</t> <t>FUS-GFP</t> and exposed to H 2 O 2 with no additive ( −), R10, or Arg. FUS-GFP (green) and anti-tyrosine hydroxylase (TH) antibody (neuron cell marker, red) were respectively detected. Scale bar denotes 10 µm. ( B ) Quantification of the area of FUS clusters in TH + neurons. Error bars represent the standard errors ( N = 20). *** and **** indicate significant differences ( p < 0.001 and p < 0.0001 for control) in one-way analysis of variance (ANOVA) with post-hoc Tukey’s multiple comparison test, respectively. $$$$ and #### indicate significant differences ( p < 0.0001 for no additive ( −) and p < 0.0001 for R10) in one-way ANOVA with post-hoc Tukey’s multiple comparison test, respectively.
Human Fus, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fus/product/Sino Biological
Average 90 stars, based on 1 article reviews
human fus - by Bioz Stars, 2026-03
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nbp1 42462 immunoprecipitation  (Novus Biologicals)
93
Novus Biologicals nbp1 42462 immunoprecipitation
Arg and poly-arg additives worked similarly for regulating <t>FUS</t> cluster formation in cultured dopaminergic neurons. ( A ) Effect of Arg and poly-Arg additives to FUS cluster formation in the cells detected by a confocal laser scanning microscopy. Cells were transfected <t>with</t> <t>FUS-GFP</t> and exposed to H 2 O 2 with no additive ( −), R10, or Arg. FUS-GFP (green) and anti-tyrosine hydroxylase (TH) antibody (neuron cell marker, red) were respectively detected. Scale bar denotes 10 µm. ( B ) Quantification of the area of FUS clusters in TH + neurons. Error bars represent the standard errors ( N = 20). *** and **** indicate significant differences ( p < 0.001 and p < 0.0001 for control) in one-way analysis of variance (ANOVA) with post-hoc Tukey’s multiple comparison test, respectively. $$$$ and #### indicate significant differences ( p < 0.0001 for no additive ( −) and p < 0.0001 for R10) in one-way ANOVA with post-hoc Tukey’s multiple comparison test, respectively.
Nbp1 42462 Immunoprecipitation, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nbp1 42462 immunoprecipitation/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
nbp1 42462 immunoprecipitation - by Bioz Stars, 2026-03
93/100 stars
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vector pcmv6 ac  (OriGene)
90
OriGene vector pcmv6 ac
Arg and poly-arg additives worked similarly for regulating <t>FUS</t> cluster formation in cultured dopaminergic neurons. ( A ) Effect of Arg and poly-Arg additives to FUS cluster formation in the cells detected by a confocal laser scanning microscopy. Cells were transfected <t>with</t> <t>FUS-GFP</t> and exposed to H 2 O 2 with no additive ( −), R10, or Arg. FUS-GFP (green) and anti-tyrosine hydroxylase (TH) antibody (neuron cell marker, red) were respectively detected. Scale bar denotes 10 µm. ( B ) Quantification of the area of FUS clusters in TH + neurons. Error bars represent the standard errors ( N = 20). *** and **** indicate significant differences ( p < 0.001 and p < 0.0001 for control) in one-way analysis of variance (ANOVA) with post-hoc Tukey’s multiple comparison test, respectively. $$$$ and #### indicate significant differences ( p < 0.0001 for no additive ( −) and p < 0.0001 for R10) in one-way ANOVA with post-hoc Tukey’s multiple comparison test, respectively.
Vector Pcmv6 Ac, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector pcmv6 ac/product/OriGene
Average 90 stars, based on 1 article reviews
vector pcmv6 ac - by Bioz Stars, 2026-03
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fus  (OriGene)
89
OriGene fus
a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and <t>FUS</t> <t>proteins</t> on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.
Fus, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
fus - by Bioz Stars, 2026-03
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flag fus  (Sino Biological)
94
Sino Biological flag fus
a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and <t>FUS</t> <t>proteins</t> on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.
Flag Fus, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flag fus/product/Sino Biological
Average 94 stars, based on 1 article reviews
flag fus - by Bioz Stars, 2026-03
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plasmid expressing human fus-myc  (Promega)
90
Promega plasmid expressing human fus-myc
a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and <t>FUS</t> <t>proteins</t> on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.
Plasmid Expressing Human Fus Myc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid expressing human fus-myc/product/Promega
Average 90 stars, based on 1 article reviews
plasmid expressing human fus-myc - by Bioz Stars, 2026-03
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human fus elisa kit  (FineTest Biotech Inc)
90
FineTest Biotech Inc human fus elisa kit
a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and <t>FUS</t> <t>proteins</t> on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.
Human Fus Elisa Kit, supplied by FineTest Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fus elisa kit/product/FineTest Biotech Inc
Average 90 stars, based on 1 article reviews
human fus elisa kit - by Bioz Stars, 2026-03
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his-tagged human fus lc gene  (GenScript corporation)
90
GenScript corporation his-tagged human fus lc gene
a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and <t>FUS</t> <t>proteins</t> on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.
His Tagged Human Fus Lc Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/his-tagged human fus lc gene/product/GenScript corporation
Average 90 stars, based on 1 article reviews
his-tagged human fus lc gene - by Bioz Stars, 2026-03
90/100 stars
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small-interfering rna against the human fus gene  (Ribobio co)
90
Ribobio co small-interfering rna against the human fus gene
FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific <t>siRNA</t> (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).
Small Interfering Rna Against The Human Fus Gene, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/small-interfering rna against the human fus gene/product/Ribobio co
Average 90 stars, based on 1 article reviews
small-interfering rna against the human fus gene - by Bioz Stars, 2026-03
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codon optimized sequence for human fus low-complexity region  (Synbio Technologies LLC)
90
Synbio Technologies LLC codon optimized sequence for human fus low-complexity region
FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific <t>siRNA</t> (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).
Codon Optimized Sequence For Human Fus Low Complexity Region, supplied by Synbio Technologies LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/codon optimized sequence for human fus low-complexity region/product/Synbio Technologies LLC
Average 90 stars, based on 1 article reviews
codon optimized sequence for human fus low-complexity region - by Bioz Stars, 2026-03
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human fus overexpression pcmv-fus vectors  (Obio Technology Corp Ltd)
90
Obio Technology Corp Ltd human fus overexpression pcmv-fus vectors
FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific <t>siRNA</t> (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).
Human Fus Overexpression Pcmv Fus Vectors, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fus overexpression pcmv-fus vectors/product/Obio Technology Corp Ltd
Average 90 stars, based on 1 article reviews
human fus overexpression pcmv-fus vectors - by Bioz Stars, 2026-03
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fus human sirna oligo duplex  (OriGene)
89
OriGene fus human sirna oligo duplex
FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific <t>siRNA</t> (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).
Fus Human Sirna Oligo Duplex, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
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Image Search Results


Arg and poly-arg additives worked similarly for regulating FUS cluster formation in cultured dopaminergic neurons. ( A ) Effect of Arg and poly-Arg additives to FUS cluster formation in the cells detected by a confocal laser scanning microscopy. Cells were transfected with FUS-GFP and exposed to H 2 O 2 with no additive ( −), R10, or Arg. FUS-GFP (green) and anti-tyrosine hydroxylase (TH) antibody (neuron cell marker, red) were respectively detected. Scale bar denotes 10 µm. ( B ) Quantification of the area of FUS clusters in TH + neurons. Error bars represent the standard errors ( N = 20). *** and **** indicate significant differences ( p < 0.001 and p < 0.0001 for control) in one-way analysis of variance (ANOVA) with post-hoc Tukey’s multiple comparison test, respectively. $$$$ and #### indicate significant differences ( p < 0.0001 for no additive ( −) and p < 0.0001 for R10) in one-way ANOVA with post-hoc Tukey’s multiple comparison test, respectively.

Journal: Scientific Reports

Article Title: Characterization of design grammar of peptides for regulating liquid droplets and aggregates of FUS

doi: 10.1038/s41598-021-86098-1

Figure Lengend Snippet: Arg and poly-arg additives worked similarly for regulating FUS cluster formation in cultured dopaminergic neurons. ( A ) Effect of Arg and poly-Arg additives to FUS cluster formation in the cells detected by a confocal laser scanning microscopy. Cells were transfected with FUS-GFP and exposed to H 2 O 2 with no additive ( −), R10, or Arg. FUS-GFP (green) and anti-tyrosine hydroxylase (TH) antibody (neuron cell marker, red) were respectively detected. Scale bar denotes 10 µm. ( B ) Quantification of the area of FUS clusters in TH + neurons. Error bars represent the standard errors ( N = 20). *** and **** indicate significant differences ( p < 0.001 and p < 0.0001 for control) in one-way analysis of variance (ANOVA) with post-hoc Tukey’s multiple comparison test, respectively. $$$$ and #### indicate significant differences ( p < 0.0001 for no additive ( −) and p < 0.0001 for R10) in one-way ANOVA with post-hoc Tukey’s multiple comparison test, respectively.

Article Snippet: GFP-tagged human FUS (#HG16569-ACG) was purchased from Sino Biological Inc.

Techniques: Cell Culture, Confocal Laser Scanning Microscopy, Transfection, Marker

a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and FUS proteins on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.

Journal: Nature

Article Title: EWS–FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma

doi: 10.1038/nature25748

Figure Lengend Snippet: a, Cell viability following etoposide treatment. Etoposide dose causing 35% lethality (LD35, dotted grey line) was used for further experiments. Mean ± s.d., n = 4 technical replicates, one-way ANOVA. b, Etoposide-induced TC32 cytotoxicity after EWS–FLI1 knockdown (siFLI1). n = 4 transfection replicates, two-tailed t-tests. c, Heatmap of damage-induced differential gene expression. d, CDK9 kinase activity inhibition by recombinant EWSR1 and FUS proteins on CDKtide or CTD substrates. n = 3 technical replicates, one-way ANOVA. e–g, Levels of phosphorylated Ser2/Ser5 RNAPII in U2OS cells with EWSR1 knockdown (e), IMR90 cells versus TC32 cells (f), and TC32 cells with EWS–FLI1 knockdown (g). h, Transcriptional activity after etoposide treatment. Centre at median, n = 100 cells, two-way ANOVA. Mean ± s.e.m., *P < 0.05, **P < 0.005.

Article Snippet: In vitro RNAPII phosphorylation assay The following purified recombinant proteins were purchased: RNAPII CTD fragment (POLR2A-1149H, Creative BioMart), EWSR1 (TP303709, Origene), FUS (TP301808, Origene) and CDK9/cyclin T1 (PV4131, ThermoFisher Scientific).

Techniques: Transfection, Two Tailed Test, Expressing, Activity Assay, Inhibition, Recombinant

FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific siRNA (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).

Journal: Oncology Reports

Article Title: MicroRNA-141 and its associated gene FUS modulate proliferation, migration and cisplatin chemosensitivity in neuroblastoma cell lines

doi: 10.3892/or.2016.4640

Figure Lengend Snippet: FUS downregulation inhibits proliferation and cell cycle progression in neuroblastoma cell lines. IMR-32 and SH-SY5Y cells were transfected with FUS-specific siRNA (FUS-siRNA) or control siRNA (C-siRNA). (A) qRT-PCR was used to compare the endogenous FUS expression between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (B) An MTT assay was used to compare cell proliferation ability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05). (C) A cell cycle assay was used to compare the percentages of G0/G1-, S- and G2/M-phase NB cells transfected with FUS-siRNA and those of the NB cells transfected with C-siRNA ( * P<0.05).

Article Snippet: A small-interfering RNA (siRNA) against the human FUS gene (FUS-siRNA), and its control siRNA (C-siRNA) were purchased from RiboBio.

Techniques: Transfection, Control, Quantitative RT-PCR, Expressing, MTT Assay, Cell Cycle Assay

FUS downregulation inhibits migration and increases cisplatin sensitivity in neuroblastoma cell lines. (A) A wound healing assay was used to compare the migratory capability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA. Phase contrast images were captured at 0 and 24 h after initial wounding. (B) After siRNA transfection, IMR-32 and SH-SY5Y cells were incubated with various concentrations of cisplatin at 0, 10, 25, 50 or 100 µ M for 24 h. An MTT assay was used to compare the cisplatin sensitivity between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05).

Journal: Oncology Reports

Article Title: MicroRNA-141 and its associated gene FUS modulate proliferation, migration and cisplatin chemosensitivity in neuroblastoma cell lines

doi: 10.3892/or.2016.4640

Figure Lengend Snippet: FUS downregulation inhibits migration and increases cisplatin sensitivity in neuroblastoma cell lines. (A) A wound healing assay was used to compare the migratory capability between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA. Phase contrast images were captured at 0 and 24 h after initial wounding. (B) After siRNA transfection, IMR-32 and SH-SY5Y cells were incubated with various concentrations of cisplatin at 0, 10, 25, 50 or 100 µ M for 24 h. An MTT assay was used to compare the cisplatin sensitivity between NB cells transfected with FUS-siRNA and NB cells transfected with C-siRNA ( * P<0.05).

Article Snippet: A small-interfering RNA (siRNA) against the human FUS gene (FUS-siRNA), and its control siRNA (C-siRNA) were purchased from RiboBio.

Techniques: Migration, Wound Healing Assay, Transfection, Incubation, MTT Assay